Skip to main content
. Author manuscript; available in PMC: 2016 Oct 27.
Published in final edited form as: Biochemistry. 2015 Oct 9;54(42):6462–6474. doi: 10.1021/acs.biochem.5b00532

Figure 10.

Figure 10

Effect of Cys to Ser mutations upon Fe content and phosphatase activity of Asp1371–920. Panel A: “As-purified” wild-type (“WT”) Asp1371–920, or the indicated Cys to Ser mutants (residue number noted on the X-axis), were assayed for Fe content (gray bars) or Pi release (black bars) from 10 μM 1,5-InsP8 substrate. Panel B: Regression analysis of Fe content versus Pi release for each of the constructs described in panel A (wild-type is the open circle).