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. Author manuscript; available in PMC: 2016 Oct 27.
Published in final edited form as: Biochemistry. 2015 Oct 9;54(42):6462–6474. doi: 10.1021/acs.biochem.5b00532

Figure 9.

Figure 9

HPLC analysis of 5-InsP7 1-kinase activity of full-length Asp1 and the Asp1H807A mutant. Panel A shows HPLC analysis of of 5-[3H]InsP7 phosphorylation to 1,5-[3H]InsP8 after full-length Asp1 (8.6 μg/mL) was incubated with 10 μM 5-[3H]InsP7 in kinase-assay buffer (see Materials and Methods) at 25 °C for 30 min, either in “as-purified” enzyme (circles) or after reconstitution with Fe3+,2+ and S2− (squares). Panel B shows HPLC analysis of similar kinase assays for the as-purified, full-length Asp1H807A mutant (8.6 μg/mL) incubated with 10 μM 5-[3H]InsP7 for either 0 min (triangles) or 30 min (circles).