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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Glycoconj J. 2015 May 10;32(7):515–529. doi: 10.1007/s10719-015-9593-7

Figure 2. Metabolism of ManNDAz and Ac4ManNDAz by MDA-MB-231 cells.

Figure 2

MDA-MB-231 cells were cultured with 4 mM ManNDAz or 100 μM Ac4ManNDAz for 48 h. (a) Intracellular and membrane sialic acids were measured by fractionation, DMB derivatization, and HPLC analysis with fluorescence detection. Analysis was performed in biological duplicate with error bars showing standard deviation of the mean. (b) Intracellular CMP-sialic acids were measured by HPAEC with UV detection at 260 nm.