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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Glycoconj J. 2015 May 10;32(7):515–529. doi: 10.1007/s10719-015-9593-7

Figure 6. BJAB K20 cells poorly metabolize protected sialic acids.

Figure 6

(a) BJAB K20 cells were cultured with indicated molecules for 24 h and intracellular levels of CMP-sialic acids were quantified by HPAEC-UV. Analysis was performed in biological duplicate with error bars showing standard deviation of the mean. (b) BJAB K20 cells were cultured with 100 μM Ac4ManNAc or 100 μM Ac5-1-OMe-Neu5Ac for 6 or 24 h. Intracellular CMP-Neu5Ac was quantified by HPAEC-UV. Analysis was performed in biological duplicate with error bars showing standard deviation of the mean. (c) BJAB K20 cells were cultured with indicated molecules for 48 h. Cell surface α2–3-linked sialic acid was measured by flow cytometry using biotinylated MAL II lectin and APC-streptavidin. Data presented are a single trial representative of replicate experiments.