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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Glycoconj J. 2015 May 10;32(7):515–529. doi: 10.1007/s10719-015-9593-7

Figure 7. Ac5-1-OMe-Neu5Ac persists intracellularly.

Figure 7

(a) LC-MS/MS detection of Ac5-1-OMe-Neu5Ac in Jurkat cells cultured with ethanol control, Ac4ManNDAz, or Ac5-1-OMe-Neu5Ac for 24 h. BJAB K88 or Jurkat cells were cultured with Ac5-1-OMe-Neu5Ac for 24 h (b, c) or 48 h (d). Intracellular Ac5-1-OMe-Neu5Ac was quantified by MRM with LC-MS/MS. Insets show calibration curves for synthetic Ac5-1-OMe-Neu5Ac. Data presented are the average of duplicate trials with error bars depicting the standard deviation.