Table 1. Cell surface incorporation of SiaDAz.
Cells were cultured with 100 μM Ac4ManNDAz. Membrane-associated Neu5Ac and SiaDAz were quantified. The fraction [SiaDAz]/([SiaDAz] +[Neu5Ac]) is reported as percent cell surface incorporation of SiaDAz.
| Cell Line | Cell type | Media | % cell surface incorporation of SiaDAz |
|---|---|---|---|
| BJAB K88 | B cell lymphoma | Serum Normal | 34 |
| BJAB K20 | B cell lymphoma | Serum Normal | 50 |
| BJAB K20 | B cell lymphoma | Serum Free | 100 |
| Jurkat | T cell lymphoma | Serum Normal | 65 |
| Daudi | B cell lymphoma | Serum Normal | 36 |
| HeLa | cervical carcinoma | Serum Normal | 0 |
| SW48 | colorectal carcinoma | Serum Normal | 10 |
| K562 | myelogenous leukemia | Serum Normal | 0 |
| HEK293T | kidney adenocarcinoma | Serum Normal | 13 |
| HEK293 | kidney adenocarcinoma | Serum Normal | 30 |
| T84 | colorectal carcinoma | Serum Normal | 7 |
| T84 | colorectal carcinoma | Serum Free | 15 |
| Caco-2 | colorectal carcinoma | Serum Normal | 39 |
| MDA-MB-231 | mammary carcinoma | Serum Normal | 42 |
| SK-N-SH | neuroblastoma | Serum Normal | 15 |
| SH-SY | neuroblastoma | Serum Normal | 0 |
| PC-3 | prostate cancer | Serum Normal | 0 |