Figure EV2. Validation of DDSR1-regulated mRNAs by additional siRNAs.
- Human fibroblasts cells were transfected with siNS or siDDSR1#2, and 72 h later, the transcript levels of the genes indicated were measured.
- Human fibroblasts cells were transfected with control locked nucleic acid antisense oligonucleotides or an antisense oligonucleotide against DDSR1, and 48 h later, the transcript levels of the genes indicated were measured.
Data information: RNA samples were analyzed by quantitative RT–PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change compared to control cells (P < 0.05). Statistical comparisons were made using Student’s t-test.