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. Author manuscript; available in PMC: 2015 Nov 11.
Published in final edited form as: Cold Spring Harb Protoc. 2011 Sep 1;2011(9):10.1101/pdb.prot065474 pdb.prot065474. doi: 10.1101/pdb.prot065474

FIGURE 1.

FIGURE 1

Schematic of thinned skull preparation. A head immobilization device including a custom-built plate and a skull holder is used for reducing movement artifacts during imaging (A). The skull holder/razor blades are glued onto the mouse skull such that the region of interest is exposed in the center of the circular hole of the razor blades. The skull holder/razor blades are then tightened on the aluminum blocks of the custom-built plate. A circular area of skull (typically ~0.5–1mm in diameter, marked with a blue circle) over the region of interest is shaved (B). The thinnest region (marked with a pink circle) for TPLSM imaging is ~20 µm in thickness and ~200 µm in diameter.

FIGURE 1.

FIGURE 1

Schematic of thinned skull preparation. A head immobilization device including a custom-built plate and a skull holder is used for reducing movement artifacts during imaging (A). The skull holder/razor blades are glued onto the mouse skull such that the region of interest is exposed in the center of the circular hole of the razor blades. The skull holder/razor blades are then tightened on the aluminum blocks of the custom-built plate. A circular area of skull (typically ~0.5–1mm in diameter, marked with a blue circle) over the region of interest is shaved (B). The thinnest region (marked with a pink circle) for TPLSM imaging is ~20 µm in thickness and ~200 µm in diameter.