Skip to main content
. Author manuscript; available in PMC: 2015 Nov 11.
Published in final edited form as: Cold Spring Harb Protoc. 2011 Sep 1;2011(9):10.1101/pdb.prot065474 pdb.prot065474. doi: 10.1101/pdb.prot065474

FIGURE 3.

FIGURE 3

Transcranial TPLSM imaging of enhanced GFP (eGFP)-labeled microglia vasculature within the mouse cerebellum. Two-dimensional projections of a 3D z-stack from the cerebellum (40×, digital zoom=1) of a mouse harboring a single copy of the CX3CR1-eGFP allele driving eGFP expression in a subset of myeloid cells, including central nervous system resident parenchymal microglia imaged 24 h apart (A,B). The cerebellar vasculature has been labeled in red by intravenous injection of a rhodamine-dextran conjugate solution. The stacks are 6 µm in depth (2-µm step size) and are representative of a section of cortex spanning 40–46 µm below the dural surface. eGFP-labeled microglia retain their characteristic highly branched morphology, indicative of a resting state.