Atg8 is conjugated to PMME in cells lacking Opi3. Membrane‐enriched fractions were isolated from
opi3Δ cells expressing His–FLAG–Atg8, solubilized, and subjected to tandem affinity chromatography as in Fig
5C and D. Negative‐ion MALDI‐MS/MS spectra of the C‐terminal peptide of His–FLAG–Atg8 from
opi3Δ cells (c) was compared with those of
in vitro‐generated Atg8–PE (a) and Atg8–PMME (b).