Atg8 lipidation is partially reduced by choline treatment in
opi3‐null cells overexpressing Atg32. Wild‐type and
opi3Δ cells expressing mito‐DHFR–mCherry and a chromosomally encoded Atg32–HA were transformed with two Atg32–HA‐expressing plasmids, cultured in the presence or absence of choline, and analyzed as in Fig
7A. Pgk1 was monitored as a loading control.