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. 2015 Nov 11;10(11):e0142182. doi: 10.1371/journal.pone.0142182

Fig 1. Effects of Prestwick Chemical Library® compounds on guanine nucleotide binding by Ras-related GTPases.

Fig 1

Tests of 888 compounds identified select NSAIDs as active against guanine nucleotide binding measured as the % of BODIPY-GTP bound in the presence of added compound relative to DMSO treated controls. Dose response assays on eight Ras-related identified R-naproxen as having selective activity against multiple Rac and Cdc42 GTPases. CID2950007/ML141 is a characterized Cdc42 selective inhibitor that served as a positive control. Multiplex dose response assays conducted on eight Ras-related GTPases, under the same conditions as primary screens with Z’ ranging from 0.62 to 0.84, identified R-naproxen as having selective activity against multiple Rac and Cdc42 GTPases.