Table 2. Primers and probes used in this study (Tm = melting temperature in °C).

Name	Description	Sequence	Tm (°C)	Source
RT-18S-F2	18S forward primer for real-time PCR	5'-ACCGCCCTAGTTCTAACCGTAAA	62.9	Barr et al. [22, 27]
RT-18S-R2	18S reverse primer for real-time PCR	5'-CCGCCGAGCCATTGTAGTAA	60.5	Barr et al. [22]
RT-18S-P2	18S real-time PCR probe	5'-Quasar 670-TGTCATCTAGCGATCCGCCGA-BHQ-2	63.2	Barr et al. [22]/This study
ITSF	5.8S-ITS2-28S	5'-TTGAACATCGACATTTCGAACGCAC	64.1	Barr et al. [22, 27]
ITSR	5.8S-ITS2-28S	5'-TCCTCCGCTTATTGATATGC	56.4	Barr et al. [22, 27]
425F	ITS2 forward primer for real-time PCR	5'-ACAAYACCAGAGGGGGTYGC	60.5–64.6	This study
568R	ITS2 reverse primer for real-time PCR	5'-CGTCGATGCGCTCTTCGG	60.8	This study
QP-Harm-ITS2-P8	ITS2 real-time PCR probe for H. armigera	5'-FAM-TGTCGTCCGYTTTAGCGTGAGAC-BHQ-1	64.6–66.6	This study
RT-ITS-zea	ITS2 real-time PCR probe for H. zea	5'-HEX-CAACGCCATTAGTAGGCGGACTC-BHQ-1	66.6	This study