Table 3. Cq values for each probe resulting from testing different real-time PCR master mixes with the triplex assay.
ANOVA statistics for comparisons of each data set are provided in the last row of the table.
| Quasar 670 (Control)—Cq values | FAM (H. armigera)—Cq values | HEX (H. zea)—Cq values | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Min. | Max. | Mean | ± SD | Min. | Max. | Mean | ± SD | Min. | Max. | Mean | ± SD | |
| Roche LightCycler 480 Probes Master | 14.11 | 18.44 | 15.77 | 0.92 | 14.22 | 23.06 | 16.99 | 1.45 | 11.81 | 15.75 | 13.26 | 0.81 |
| Takara Premix Ex Taq (Perfect Real Time) | 12.44 | 23.94 | 15.32 | 2.71 | 13.20 | 23.62 | 16.31 | 1.85 | 10.54 | 20.27 | 13.02 | 2.28 |
| Bio-Rad iTaq Universal Probes Supermix | 13.80 | 23.68 | 16.48 | 2.55 | 12.88 | 22.17 | 15.95 | 1.61 | 11.16 | 19.64 | 13.55 | 2.21 |
| Statistics: | df = 2 | F ratio = 4.25 | P = 0.015 | Statistics: | df = 2 | F ratio = 3.29 | P = 0.041 | Statistics: | df = 2 | F ratio = 1.64 | P = 0.196 | |