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. 2015 Nov 11;10(11):e0142902. doi: 10.1371/journal.pone.0142902

Fig 2. Effect of simvastatin on insulin secretion mediated by ATP dependent potassium channels, L-type voltage-gated calcium channels, acetylcholine, and phorbol myristate acetate in MIN6 β-cells.

Fig 2

The effect of simvastatin (Simva, 14.3 μM) on insulin secretion stimulated by tolbutamide (Tol, 100 μM) and potassium chloride (KCl, 40 mM) at 5.5 mM (A) and 16.7 mM glucose concentrations (B); the effect of simvastatin and nifedipine (Nif, 5 μM) on insulin secretion at 5.5 mM (C) and 16.7 mM (D) glucose concentrations; the effect of simvastatin on insulin secretion stimulated by acetylcholine (Ach, 10 μM) at 5.5 mM glucose concentration (E); and the effect of simvastatin on insulin secretion stimulated by phorbol myristate acetate (PMA, 0.5 μM) at 5.5 mM glucose concentration (F); and the effect of simvastatin (14.3 μM) on the PKC activity in MIN6 β-cells at 5.5 and 16.7 mM glucose concentration (G). Insulin secretion was normalized with protein concentration, data are mean ± SEM relative to control (100%). p values were calculated with the Mann-Whitney test, *p<0.05, **p<0.01 compared to control. The number of replicates was 6 for A and E, 8 for B, C, and D, 4 for F, and 2–4 for G.