Table 1. Substrate specificity of the purified BacA protein.
The BacA activity was tested as described in Materials and Methods in the presence of 1 mM CaCl2, using the listed compounds at a 50 μM concentration. Results are expressed as mean ± standard deviation of three individual experiments. ND, no detectable activity, when up to 2 μg of pure BacA protein was used per assay.
| Compound | BacA phosphatase activity (μmol.min-1.mg-1) |
|---|---|
| C55-PP | 11.3 ± 1.3 |
| C15-PP | 10.3 ± 0.9 |
| C5-PP | ND |
| Diacyl(C8)glycerol-PP | 5.5 ± 0.4 |
| Phosphatidylglycerol-P | ND |
| Phosphatidic acid | ND |