Fig 1. Characterization of CD8+ T cells at 28 days postinfection upon Ara-LAM treatment in Leishmania donovani infected BALB/c mice.

(A) CD8⁺ T from differently treated BALB/c mice 28 days postinfection were subjected to FACS analyis to check the expression of CD25⁺IL12R⁺, CD25⁺CD28⁺, CD25⁺IFN-γR⁺ cells. Data are from one of three representative experiments. (B) In separate set of experiment, CD8⁺ T cells from differently treated mice group were isolated and cultured in presence of plate-bound anti-CD3ε mAbs (5μg/mL) and CD28 (1μg/mL) and expresion of perforin and granzyme-B was done by conventional RT PCR. Data are from one of three representative experiments.