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. 2015 Nov 11;10(11):e0142800. doi: 10.1371/journal.pone.0142800

Table 1. Sequences of the PCR primers.

Gene Sequences
TLR2 FP 5’- TCTGGGCAGTCTTGAACATTT -3’
RP 5’- AGAGTCAGGTGATGGATGTCG -3’
IFN-γ FP 5’- AGCTCTTCCTCATGGCTGTTTC -3’
RP 5’- TGTTGCTGATGGCCTGATTGT -3’
Perforin FP 5’- CTGAGCGCCTTTTTGAAGTC -3’
RP 5’- AAGGTAGCCAATTTTGCAGC -3’
Granzyme-B FP 5’- CTCTCGAATAAGGAAGCCCC -3'
RP 5’- CTGACCTTGTCTCTGGCCTC -3'
T-bet FP 5’- CCTCTTCTATCCAACCAGTATC -3’
RP 5’- CTCCGCTTCATAACTGTGT -3’
IFN-γ promoter FP 5’- GAGAAATTCACATTACAAGGGC -3’
RP 5’- TTAAGATGGTGACAGATAGGTGG -3’
Perforin promoter FP 5’- GTACTAGCCTGCTCAAACCT -3’
RP 5’- CTAATCACAGTGTCCCATGAG -3’
Granzyme promoter FP 5’- ATGCTCCTGATTACCCTCAC -3’
RP 5’- CAGAGAACCACCACTTACAG -3’
TLR-2 siRNA FP 5’-AAAGAGAAAGTACTTACTGCACCTGTCTC-3’
RP 5’- AATGCAGTAAGTACTTTCTCTCCTGTCTC -3’
T-bet siRNA FP 5’- AAACAAACATCCTGTAATGGCCCTGTCTC -3’
RP 5’-AAGCCATTACAGGATGTTTGTCCTGTCTC-3’
GAPDH FP 5’- GTTGTCTCCTGCGACTTCAACA -3’
RP 5’- TCTCTTGCTCAGTGTCCTTGCT -3’