Table 1. List of primary antibodies used for fluorescent IHC on coronal SC sections.

Primary antibody	Labels	Incubation time and dilution	Reference
Polyclonal rabbit anti-eGFP	eGFP	24 h, 1:10,000	In house made [39]
Monoclonal mouse anti-GFAP	(Reactive) astrocytes	24 h, 1:1,000	G3893, Sigma-Aldrich, MO, USA
Monoclonal mouse anti-NeuN	Neuronal nuclei	24 h, 1:200	MAB377, Millipore, Germany
Polyclonal rabbit anti-BDNF	Pro- and mBDNF	48 h, 1:500	sc-546, Santa Cruz Biotechnology, TX, USA

Key: IHC, immunohistochemistry; SC, superior colliculus; eGFP, enhanced green fluorescent protein; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei; BDNF, brain-derived neurotrophic factor; mBDNF, mature form of brain-derived neurotrophic factor.