Table 1. Mutation spectra induced by integration of pvITG, pvIT1x8oG, pvIT2x8oG, and pvINT2x8oG.
| Targeting vector | Single point mutation at 8-oxoG a | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell | TK revertants analyzed | DNA adducts-integrated revertants | T | C | A | Del b | Ins c | Tandem mutations d | Non-targeted e | Total mutation | ND f | |
| pvITG | TSCER122 | 264 | 236 (100%) | 0 | 0 | 0 | 0 | 0 | 0 | 3 (1.3%) | 3 (1.3%) | 1 |
| XPA KO | 260 | 227 (100%) | 0 | 0 | 0 | 0 | 0 | 0 | 3 (1.3%) | 3 (1.3%) | 0 | |
| pvIT1x8oG | TSCER122 | 461 | 422 (100%) | 15 (3.6%) | 5 (1.2%) | 1 (0.24%) | 3 (0.71%) | 0 | 1 (0.24%) | 9 (2.1%) | 34 (8.1%) | 3 |
| XPA KO | 538 | 472 (100%) | 12 (2.5%) | 11 (2.3%) | 3 (0.64%) | 1 (0.21%) | 0 | 0 | 9 (1.9%) | 36 (7.6%) | 3 | |
| pvIT2x8oG | TSCER122 | 713 | 649 (100%) | 19 (2.9%) | 11 (1.7%) | 2 (0.31%) | 0 | 1 (0.15%) | 8 (1.2%) | 7 (1.1%) | 48 (7.4%) | 4 |
| XPA KO | 816 | 703 (100%) | 31 (4.4%) | 6 (0.85%) | 5 (0.71%) | 2 (0.28%) | 1 (0.14%) | 21 (3.0%) | 20 (2.8%) | 86 (12%) | 3 | |
| pCl-XPA g | 541 | 471 (100%) | 13 (2.8%) | 5 (1.1%) | 3 (0.64%) | 0 | 0 | 7 (1.5%) | 7 (1.5%) | 35 (7.4%) | 0 | |
| pvINT2x8oG | TSCER122 | 641 | 592 (100%) | 35 (5.9%) | 10 (1.7%) | 0 | 2 (0.34%) | 1 (0.17%) | 12 (2.0%) | 8 (1.4%) | 68 (11%) | 0 |
| XPA KO | 633 | 539 (100%) | 20 (3.7%) | 11 (2.0%) | 3 (0.56%) | 0 | 1 (0.19%) | 14 (2.6%) | 14 (2.6%) | 63 (12%) | 0 | |
a A single-base substitution, one-base insertion, or one-base deletion detected at an 8-oxoG. In the cases of pvIT2x8oG and pvINT2x8oG, single point mutation at 8-oxoG indicates a mutation detected at one 8-oxoG site.
b One-base deletion.
c One-base insertion.
d Multiple base substitutions, deletions, and/or insertions detected at sites, including 8-oxoG, in 12 bp around the BssSI site.
e Mutations found at sites other than 8-oxoG loci.
f Not detectable because of too low signal strength.
g XPA KO + pCl-XPA cells.