Table 2. Spectra of single and tandem mutations induced by integration of pvIT1x8oG, pvIT2x8oG, and pvINT2x8oG.
| pvIT1x8oG | pvIT2x8oG | pvINT2x8oG | ||||||
|---|---|---|---|---|---|---|---|---|
| 5′-TCCCAC8AGGCT-3′ b | 5′-TCCCAC8A8GCT-3′ | 5′-TCCCACGAGGCT-3′ | ||||||
| 3′-AGGGTGCTCCGA-5′ | 3′-AGGGTGCTCCGA-5′ | 3′-AGG8T8CTCCGA-5′ | ||||||
| Mutation a | TSCER122 | XPA KO | TSCER122 | XPA KO | pCl-XPA c | TSCER122 | XPA KO | |
| 5′-TCCCACGAGGCT (original) | ||||||||
| Single | 5′-TCCCAC T AGGCT | 15 (3.6%) | 12 (2.5%) | 9 (1.4%) | 14 (2.0%) | 4 (0.85%) | 0 | 0 |
| 5′-TCCCAC C AGGCT | 5 (1.2%) | 11 (2.3%) | 8 (1.2%) | 3 (0.43%) | 3 (0.64%) | 0 | 0 | |
| 5′-TCCCAC A AGGCT | 1 (0.24%) | 3 (0.64%) | 1 (0.15%) | 4 (0.57%) | 3 (0.64%) | 0 | 0 | |
| 5′-TCCCAC Δ AGGCT | 3 (0.71%) | 1 (0.21%) | 0 | 2 (0.28%) | 0 | 0 | 0 | |
| 5′-TCCCACGA TGCT | 0 | 0 | 10 (1.5%) | 17 (2.4%) | 9 (1.9%) | 0 | 0 | |
| 5′-TCCCACGA CGCT | 0 | 0 | 3 (0.46%) | 3 (0.43%) | 2 (0.42%) | 0 | 0 | |
| 5′-TCCCACGA AGCT | 0 | 0 | 1 (0.15%) | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCCAC C GAGGCT | 0 | 0 | 1 (0.15%) | 0 | 0 | 0 | 0 | |
| 5′-TCCCACGAG GGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCA ACGAGGCT | 0 | 0 | 0 | 0 | 0 | 2 (0.34%) | 8 (1.5%) | |
| 5′-TCCG ACGAGGCT | 0 | 0 | 0 | 0 | 0 | 5 (0.84%) | 2 (0.37%) | |
| 5′-TCCT ACGAGGCT | 0 | 0 | 0 | 0 | 0 | 0 | 1 (0.19%) | |
| 5′-TCCΔ ACGAGGCT | 0 | 0 | 0 | 0 | 0 | 2 (0.34%) | 0 | |
| 5′-TCCCA A GAGGCT | 0 | 0 | 0 | 0 | 0 | 33 (5.6%) | 12 (2.2%) | |
| 5′-TCCCA G GAGGCT | 0 | 0 | 0 | 0 | 0 | 5 (0.84%) | 9 (1.7%) | |
| 5′-TCCCA T GAGGCT | 0 | 0 | 0 | 0 | 0 | 0 | 2 (0.37%) | |
| 5′-TCCCAC C GAGGCT | 0 | 0 | 0 | 0 | 0 | 0 | 1 (0.19%) | |
| 5′-TCCCAC G GAGGCT | 0 | 0 | 0 | 0 | 0 | 1 (0.17%) | 0 | |
| Total of single | 24 (5.7%) | 27 (5.7%) | 33 (5.1%) | 45 (6.4%) | 21 (4.5%) | 48 (8.1%) | 35 (6.5%) | |
| Tandem | 5′-TCCCAC T A TGCT | 0 | 0 | 3 (0.46%) | 8 (1.1%) | 2 (0.42%) | 0 | 0 |
| 5′-TCCCAC C A TGCT | 0 | 0 | 1 (0.15%) | 3 (0.43%) | 2 (0.42%) | 0 | 0 | |
| 5′-TCCCAC T A CGCT | 0 | 0 | 1 (0.15%) | 0 | 0 | 0 | 0 | |
| 5′-TCCCAC C A CGCT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 5′-TCCCAC T A AGCT | 0 | 0 | 1 (0.15%) | 0 | 0 | 0 | 0 | |
| 5′-TCCCAC A A TGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCCACG TTGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCCACG GTGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCCAC TTTGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TTCCAC T A TGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCCAC CC AGGGT | 0 | 0 | 0 | 0 | 1 (0.21%) | 0 | 0 | |
| 5′-TCCCACGA AACT | 0 | 0 | 0 | 0 | 1 (0.21%) | 0 | 0 | |
| 5′-TCCCACGA TACT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCCA T GA CGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCΔ AC T AGGCT | 1 (0.24%) | 0 | 0 | 0 | 0 | 0 | 0 | |
| 5′-TCCΔ ACGA TGCT | 0 | 0 | 0 | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCΔ ACGA CGCT | 0 | 0 | 1 (0.15%) | 0 | 0 | 0 | 0 | |
| 5′-TCCCA AΔΔ GGCT | 0 | 0 | 1 (0.15%) | 1 (0.14%) | 0 | 0 | 0 | |
| 5′-TCCCA TΔΔΔGCT | 0 | 0 | 0 | 1 (0.14%) | 1 (0.21%) | 0 | 0 | |
| 5′-TCCA A A GAGGCT | 0 | 0 | 0 | 0 | 0 | 8 (1.4%) | 6 (1.1%) | |
| 5′-TCCA A G GAGGCT | 0 | 0 | 0 | 0 | 0 | 1 (0.17%) | 4 (0.74%) | |
| 5′-TCCG A A GAGGCT | 0 | 0 | 0 | 0 | 0 | 2 (0.34%) | 2 (0.37%) | |
| 5′-TCCΔ A A GAGGCT | 0 | 0 | 0 | 0 | 0 | 0 | 1 (0.19%) | |
| 5′-TCCGCA GAGGCT | 0 | 0 | 0 | 0 | 0 | 1 (0.17%) | 0 | |
| 5′-TCCCA G GA AGGT | 0 | 0 | 0 | 0 | 0 | 0 | 1 (0.19%) | |
| Total of tandem | 1 (0.24%) | 0 (0%) | 8 (1.2%) | 21 (3.0%) | 7 (1.5%) | 12 (2.0%) | 14 (2.6%) | |
a Underlined sequences are observed mutations. “Δ” indicates one-base deletion.
b “8” in the sequence indicates 8-oxoG.
c XPA KO + pCl-XPA cells.