Figure 4.

Cells are more sensitive to FtsZ_L169R levels compared to FtsZ_WT. (A) Representative DIC micrographs of indicated strains grown to mid-exponential phase in the presence of indicated sodium salicylate concentrations to overexpress the given ftsZ allele. Scale as in Figure 1A. (B) Immunoblot of FtsZ protein levels from mid-exponential cultures of WT or ftsZ_L169R cells in the presence of the indicated sodium salicylate concentrations, with estimated relative band intensities. Uninduced and 1 μM induced samples were normalized to Ponceau-S-stained loading controls as in Figure 1E. Note that induced samples required 2 and 4-fold dilution to maintain the FtsZ immunostaining in the linear range; because of this, the intensity of Ponceau staining of protein in the 5 μM samples is low.