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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Microbiol. 2015 Jul 4;97(5):988–1005. doi: 10.1111/mmi.13081

Figure 6.

Figure 6

FtsZL169R displays evidence of enhanced bundling in vitro compared to FtsZWT. (A) Coomassie-stained gel of supernatant or pellet fractions from sedimentation reactions at 30ºC containing 5 μM purified FtsZWT or FtsZL169R assembled with added components as indicated. (B) Relative rate (%) of GTP hydrolysis activity for purified FtsZWT or FtsZL169R assembled with given components at 30ºC. The FtsZWT rate in GTP is normalized to 100%. Error bars indicate standard deviation between three replicate experiments. (C) Representative electron micrographs of purified, negatively-stained FtsZWT or FtsZL169R in the presence of 1mM GDP, GTP, or GTP plus 10 mM CaCl2. Scale bars = 100 nm.

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