Figure 6.
FtsZL169R displays evidence of enhanced bundling in vitro compared to FtsZWT. (A) Coomassie-stained gel of supernatant or pellet fractions from sedimentation reactions at 30ºC containing 5 μM purified FtsZWT or FtsZL169R assembled with added components as indicated. (B) Relative rate (%) of GTP hydrolysis activity for purified FtsZWT or FtsZL169R assembled with given components at 30ºC. The FtsZWT rate in GTP is normalized to 100%. Error bars indicate standard deviation between three replicate experiments. (C) Representative electron micrographs of purified, negatively-stained FtsZWT or FtsZL169R in the presence of 1mM GDP, GTP, or GTP plus 10 mM CaCl2. Scale bars = 100 nm.