Table 3.
Plasmids used in this study
| Plasmid | Description | Source/reference |
|---|---|---|
| pKG110 | pACYC184 derivative containing the nahG promoter | J. Parkinson |
| pDH156 | pKG110-ftsZWT | This study |
| pDH155 | pKG110-ftsZL169R | This study |
| pKG116 | pKG110 derivative with stronger Shine-Dalgarno sequence | J. Parkinson |
| pDH161 | pKG116-ftsZWT | This study |
| pDH159 | pKG116-ftsZL169R | This study |
| pDSW208 | colE1 plasmid with slightly weakened Ptrc promoter | (Weiss et al., 1999) |
| pDSW210 | colE1 plasmid with significantly weakened Ptrc promoter | (Weiss et al., 1999) |
| pDSW210F | pWM2784 (pDSW210 with N-terminal flag epitope) | (Shiomi and Margolin, 2007b) |
| pWM2785 | pDSW210F-ftsAWT | (Shiomi and Margolin, 2007a) |
| pWM2787 | pDSW210F-ftsAR286W | (Shiomi and Margolin, 2007a) |
| pDSW208F | pDSW208 with N-terminal flag epitope | (Shiomi et al., 2008) |
| pWM3239 | pDSW208F-ftsAW408E | This study |
| pET11a | Expression vector | Novagen |
| pWM971 | pET11a-ftsZ | H. Erickson |
| pDH160 | pET11a-ftsZL169R | This study |