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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dev Biol. 2015 Aug 5;407(1):90–102. doi: 10.1016/j.ydbio.2015.08.004

Figure 2. Treatment with rapamycin inhibits mTORC1 activity without affecting AKT.

Figure 2

(A-F) Immunostaining of testis sections from mice treated with vehicle (A, C, and E) or rapamycin (B, D, and F) and euthanized at P4. Sections were stained with anti- phosphorylated RPS6 (A and B), anti- phosphorylated EIF4EBP1 (C and D), or total FOXO1 (E and F). F-actin was stained with phalloidin (red) to visualize testis cords. Scale bar = 50 μM.