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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dev Biol. 2015 Aug 5;407(1):90–102. doi: 10.1016/j.ydbio.2015.08.004

Figure 3. MTORC1 is required for postnatal expansion of the germ cell population.

Figure 3

(A-L) Immunostaining of testis sections from mice treated with vehicle (A, C, E, G, I, and K) or rapamycin (B, D, F, H, J, and L) and euthanized at P4 (A, B, E, F, I, and J) or P8 (C, D, G, H, K, and L). Sections were stained with anti-DDX4 (A-D), anti-cleaved PARP1 (E-H), or double labeled with anti-BrdU (green, I-L) and anti-DDX4 (red, I-L). F-actin was stained with phalloidin (red, A-D or blue, E-H). Quantitation of the number of DDX4+(M and N) or the number of BrdU+/DDX4+ (O and P) cells from testes treated starting at P1 with vehicle or rapamycin and then euthanized at P4 (M and O) or at P8 (N and P). Asterisks indicate statistical significance with P≤0.01. Scale bar = 50 μM.