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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Microbiol. 2015 Jun 12;97(5):832–843. doi: 10.1111/mmi.13067

Figure 1. SodCI binds to diaminopimelic acid-containing murein sacculus.

Figure 1

A) Purified SodCI, PhoA or monomeric SodCI (mSodCI) was incubated with or without purified Salmonella peptidoglycan sacculus (PGN). Supernatant (S) after 1st centrifugation (100,000×g) was saved. The remaining pellet was washed gently with buffer and another supernatant (W) and pellet (P) were obtained after 2nd centrifugation (100,000×g). All fractions were brought to the same volume and proteins were separated via PAGE. His-tagged proteins were detected by Western blot. B) SodCI binding to purified PGN from Staphylococcus or Chitin were tested as above. C) SodCI binding to Salmonella and Bacillus subtilis PGN.