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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dev Biol. 2015 Sep 10;407(1):1–11. doi: 10.1016/j.ydbio.2015.08.012

Figure 2. Microarray approach to identify ectodermal GATA2 targets required for blood formation in the mesoderm.

Figure 2

(A) Schematic of microarray strategy and sample acquisition. Embryos were injected at the two-cell stage with GATA2 MO (40 ng), FOG RNA (500 pg) or GATA2 RNA (500 pg) and cultured to the early gastrula stage (stage 10). Ectoderm was explanted and cultured to stage 12, at which point ectodermal explants in each group were pooled and RNA was extracted for microarray analysis. (B) Genes identified by microarray analysis that are predicted to be relevant for hematopoiesis (C) qPCR analysis of gene expression in ectodermal explants isolated at stage 10 from embryos injected with GATA2 MOs or FOG RNA and cultured to stage 12. Quantification of relative gene expression (mean +/− SD) in three independent experiments is shown.