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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dev Biol. 2015 Aug 13;407(1):57–67. doi: 10.1016/j.ydbio.2015.08.008

Figure 7. Treatment with the RAR inhibitor BMS 493 disrupts growth and branching morphogenesis in ex vivo cultured SMG.

Figure 7

(A–B) Transmitted light images of two SMG isolated from a single embryo at E13.5 cultured ex vivo for 48 hours. SMG cultured on medium containing 2 μM BMS 493 (B) had less epithelial growth with fewer branches and endbuds relative to the contralateral gland grown on control medium (A). (C–D) SMG isolated at E13.0 and cultured ex vivo for 48 hours and stained with E-cadherin to reveal epithelium. BMS 493 – treated glands had fewer endbuds and shallower clefts (D) than contralateral gland grown on control medium (C). (E) Quantitation of branching morphogenesis by counting endbuds of E13.5 SMG cultured 48 hours. SMG grown on control medium had an average of 29 endbuds. Contralateral glands grown on medium containing BMS 493 had an average of 3 endbuds. N = 10 gland pairs. Error bars = Standard Deviation. Black scale bars = 200μm, white scale bars = 50 μm.