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. Author manuscript; available in PMC: 2016 Feb 5.
Published in final edited form as: Oncogene. 2015 May 11;35(5):608–620. doi: 10.1038/onc.2015.119

Figure 7.

Figure 7

Targeting OPN in human GBM-bearing mice improves survival. U87 human cell line transduced with lentiviral shIRR or shOPNh were transplanted into the brain of immunocompromised NOD-SCID mice (3×105 cells per mouse). (a) Kaplan-Meier plot of survival for mice transplanted with U87-shIRR (n = 10) and U87-shOPNh (n= 10). (b) Representative images of brain sections (hematoxylin and eosin-stained) 31 days (when U87-shIRR mice started to show clinical symptoms; panels i and ii) and 55 days after transplantation (panel iii). (c) Tumors from each group were collected at the end of the experiment and brain sections (40 nm) were immunostained with (i) OPN and (ii) Ki67, and analyzed using confocal microscopy. mCHERRY is a fluorescent reporter expressed in the lentiviral shRNA construct, DAPI = nuclei. Scale bar= 150)μm.