Figure 2. Caprin-2 protein expression in the rat PVN and SON.
Immunohistochemical analysis of Caprin-2 protein expression (green) in the rat PVN (A) and SON (B) in euhydrated and salt-loaded rats; co-localization with AVP-neurophysin II (AVP; red). Scale bar 100 µm. (C) Quantification of fluorescence signals for Caprin-2 and (D) AVP in AVP cells. Mean fluorescence intensities were quantified in 10 SON wide field fluorescence microscope images acquired from 3 rats. AVP signal was selected (above the same threshold for EU and SL samples) and mean fluorescence intensity was measured for Caprin 2 and AVP signal. Each result was corrected by subtraction of mean background intensities for each channel. Salt-loading (SL) significantly increases Caprin-2 signal in AVP cells (euhydrated, 13.68 ± 1.47; salt-loaded, 19.92 ± 0.86, p = 0.0018), but results in a significant decrease of AVP in AVP cells (euhydrated, 56.94 ± 2.439; salt-loaded 48.3 ± 1.05, p = 0.0044). **p ≤ 0.01.