Figure 7. A recapitulated in vitro system for examining effect of Caprin-2 on the metabolism of the AVP mRNA.
In order to further examine to role of Caprin-2 in AVP mRNA metabolism, we developed a recapitulated in vitro system. We overexpressed the rat AVP structural gene and full-length rat Caprin-2 in HEK293T cells, both under the control of the heterologous CMV promoter. A vector expressing eGFP was used as a control. Western blotting of triplicate independent samples showing robust expression of eGFP and Caprin-2 proteins in transduced cells.