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Complete protocol of Agrobacterium mediated transformation in cotton. (A) Germinated Seeds of VH-289, (B) embryo isolation, (C) injuring of cotton embryos with sharp blade, (D) co-culturing of injured embryos in MS broth with gene of interest (Cry2A and GTG), (E) cotton embryos are shifted to selection free MS medium plate, (F) cotton embryos shifted to test tubes with selection drug (kanamycin) for screening.
