Fig. 3. (A) Glucose transporter 4 (GLUT4) protein levels assessed by Western blot and immunofluorescence assay in the skeletal muscle of the (B) Zucker lean control (ZLC-Con), (C) non-exercised Zucker diabetic fatty (ZDF-Con), and (D) exercised Zucker diabetic fatty (ZDF-Ex) rats. Paraffin sections were incubated with GLUT4 antibody (1:1,000) and then with a fluorescein isothiocyanate-conjugated secondary antibody (1:50). Values are presented as mean±standard error of mean (immunofluorescence assay, ×400; scale bar=50 µm).
