. 2015 Oct 30;30(6):873–883. doi: 10.3904/kjim.2015.30.6.873

Table 2.

Gene name and fold change in expression in nine categories of cell damage pathways after NP40 exposure

GeneBank accession no.	Gene name	Symbol	Fold change
GeneBank accession no.	Gene name	Symbol	NP40	p value^a
Oxidative/metabolic stress
NM_003329	Thioredoxin	TXN	–1.23	0.008^b
NM_001498	Glutamate-cysteine ligase, catalytic subunit	GCLC	–1.32	0.045^b
NM_002061	Glutamate-cysteine ligase, modifier subunit	GCLM	4.02^b	0.048^b
NM_000637	Glutathione reductase	GSR	1.30^b	0.689
NM_000852	Glutathione S-transferase pi 1	GSTP1	–4.24	0.389
NM_002133	Heme oxygenase (decycling) 1	HMOX1	1.60^b	0.024^b
NM_000903	NAD(P)H dehydrogenase, quinone 1	NQO1	1.99^b	0.016^b
NM_002574	Peroxiredoxin 1	PRDX1	–1.83	0.095
NM_003330	Thioredoxin reductase 1	TXNRD1	1.84^b	0.033^b
NM_003900	Sequestosome 1	SQSTM1	–2.44	0.045^b
NM_002032	Ferritin, heavy polypeptide 1	FTH1	8.78^b	0.084
Hypoxia
NM_003376	Vascular endothelial growth factor A	VEGFA	–6.09	0.009^b
NM_001668	Aryl hydrocarbon receptor nuclear translocator	ARNT	–2.86	0.022^b
NM_004331	BCL2/adenovirus E1B 19 kDa interacting protein 3-like	BNIP3L	1.18^b	0.046^b
NM_001216	Carbonic anhydrase IX	CA9	–2.82	0.037^b
NM_000799	Erythropoietin	EPO	–2.75	0.017^b
NM_002133	Hemeoxygenase (decycling) 1	HMOX1	1.60^b	0.024^b
NM_005566	Lactate dehydrogenase A	LDHA	–1.38	0.042^b
NM_004994	Matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase)	MMP9 (Gelatinase B)	–1.25	0.048^b
NM_000602	Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1	SERPINE1 (PAI-1)	–7.17	0.000^b
NM_006516	Solute carrier family 2 (facilitated glucose transporter), member 1	SLC2A1	1.87^b	0.020^b
NM_001124	Adrenomedullin	ADM	–5.24	0.080
Cell death (apoptosis signaling)
NM_003842	Tumor necrosis factor receptor superfamily, member 10b	TNFRSF10B (DR5)	8.93^b	0.037^b
NM_001065	Tumor necrosis factor receptor superfamily, member 1A	TNFRSF1A	–1.17	0.044^b
NM_021960	Myeloid cell leukemia sequence 1 (BCL2-related)	MCL1	1.87	0.004^b
NM_003844	Tumor necrosis factor receptor superfamily, member 10a	TNFRSF10A	–2.90	0.038^b
NM_033292	Caspase 1, apoptosis-related cysteine peptidase (interleukin 1, β, convertase)	CASP1 (ICE)	–1.72	0.022^b
NM_000043	Fas (TNF receptor superfamily, member 6)	FAS	–15.12	0.081
Cell death (autophagy signaling)
NM_004849	ATG5 autophagy related 5 homolog (Saccharomyces cerevisiae)	ATG5	–1.22	0.019^b
NM_006395	ATG7 autophagy related 7 homolog (S. cerevisiae)	ATG7	–4.97	0.039^b
NM_004707	ATG12 autophagy related 12 homolog (S. cerevisiae)	ATG12	2.59^b	0.017^b
NM_003766	Beclin 1, autophagy related	BECN1	1.48^b	0.029^b
NM_000043	Fas (TNF receptor superfamily, member 6)	FAS	–15.12	0.081
NM_003565	Unc-51-like kinase 1 (Caenorhabditis elegans)	ULK1	–6.74	0.018^b
Cell death (necrosis signaling)
NM_017853	Thioredoxin-like 4B	TXNL4B	7.67^b	0.071
NM_002086	Growth factor receptor-bound protein 2	GRB2	–1.99	0.029^b
NM_001618	Poly (ADP-ribose) polymerase 1	PARP1 (ADPRT1)	–1.56	0.025^b
NM_006505	Poliovirus receptor	PVR	1.57^b	0.098
NM_003804	Receptor (TNFRSF)-interacting serine-threonine kinase 1	RIPK1	13.86^b	0.096
NM_003844	Tumor necrosis factor receptor superfamily, member 10a	TNFRSF10A	–2.90	0.038^b
NM_003842	Tumor necrosis factor receptor superfamily, member 1A	TNFRSF1A	–1.17	0.044^b
NM_000043	Fas (TNF receptor superfamily, member 6)	FAS (TNFRSF6)	–15.12	0.081
Inflammatory response
NM_002982	Chemokine (C-C motif) ligand 2	CCL2 (MCP-1)	8.16^b	0.037^b
NM_000594	Tumor necrosis factor	TNF	–11.48	0.079
NM_000567	C-reactive protein, pentraxin-related	CRP	2.43^b	0.015^b
NM_000619	Interferon, γ	IFNG	–1.45	0.015^b
NM_000575	Interleukin 1, α	IL1A	–2.36	0.046^b
NM_000600	Interleukin 6 (interferon, β2)	IL-6	8.95^b	0.032^b
NM_000584	Interleukin 8	IL-8	–2.03	0.080^b
NM_138554	Toll-like receptor 4	TLR4	–4.55	0.017^b
NM_000074	CD40 ligand	CD40LG (TNFSF5)	2.84^b	0.201
DNA damage signaling (cell cycle checkpoint/arrest)
NM_004507	HUS1 checkpoint homolog (Schizosaccharomyces pombe)	HUS1	3.30^b	0.012^b
NM_001274	CHK1 checkpoint homolog (S. pombe)	CHEK1	4.36^b	0.076
NM_007194	CHK2 checkpoint homolog (S. pombe)	CHEK2 (RAD53)	3.15^b	0.000^b
NM_004083	DNA-damage-inducible transcript 3	DDIT3 (GADD153/ CHOP)	2.90^b	0.038^b
NM_002873	RAD17 homolog (S. pombe)	RAD17	1.78^b	0.012^b
NM_004584	RAD9 homolog A (S. pombe)	RAD9A	1.44^b	0.029^b
NM_000389	Cyclin-dependent kinase inhibitor 1A (p21, Cip1)	CDKN1A (p21CIP-1WAF1)	2.33^b	0.027^b
NM_005590	MRE11 meiotic recombination 11 homolog A (S. cerevisiae)	MRE11A	1.99^b	0.019^b
NM_002485	Nibrin	NBN (NBS1)	2.45^b	0.011^b
DNA damage signaling (other responses)
NM_000051	Ataxia telangiectasia mutated	ATM	–9.50	0.061
NM_001184	Ataxia telangiectasia and Rad3 related	ATR	–7.18	0.015^b
NM_000107	Damage-specific DNA binding protein 2, 48 kDa	DDB2	–2.86	0.036^b
NM_000546	Tumor protein p53	TP53	–3.52	0.006^b
NM_002875	RAD51 homolog (S. cerevisiae)	RAD51	–11.56	0.038^b
NM_001924	Growth arrest and DNA-damage-inducible, α	GADD45A	1.05^b	0.026^b
NM_004628	Xerodermapigmentosum, complementation group C	XPC	–1.04	0.023^b
Heatshock proteins/unfolded protein response
NM_001675	Activating transcription factor 4 (tax-responsive enhancer element B67)	ATF4	3.71^b	0.019^b
NM_007348	Activating transcription factor 6	ATF6	1.72^b	0.018^b
NM_004381	Activating transcription factor 6 β	ATF6B	2.15^b	0.029^b
NM_014417	BCL2 binding component 3	BBC3	1.09^b	0.041^b
NM_001196	BH3 interacting domain death agonist	BID	2.32^b	0.050^b
NM_006260	DnaJ (Hsp40) homolog, subfamily C, member 3	DNAJC3	2.73^b	0.013^b
NM_004083	DNA-damage-inducible transcript 3	DDIT3	–2.90	0.007^b
NM_004343	Calreticulin	CALR	–1.08	0.040^b
NM_001017963	Heat shock protein 90 kDa α (cytosolic), class A member 1	HSP90AA1	1.15^b	0.048^b
NM_003299	Heat shock protein 90 kDa β (Grp94), member 1	HSP90B1 (TRA1)	–5.85	0.096
NM_002154	Heat shock 70kDa protein 4	HSPA4 (HSP70)	26.10^b	0.049^b
NM_005347	Heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)	HSPA5 (GRP78)	–2.31	0.023^b
NM_014278	Heat shock 70kDa protein 4-like	HSPA4L	3.00^b	0.015^b

NP40, nonyl phenoxypolyethoxylethanol; NADPH, nicotinamide adenine dinucleotide phosphate; BCL2, B cell lymphoma 2; TNFRSF, tumor necrosis factor receptor superfamily; TNF, tumor necrosis factor; ATG, autophagy protein; ADP, adenosine diphosphate; CHK, checkpoint kinase.

The p values are calculated based on a Student t test of the replicate 2^ (- Delta Ct) values for each gene in the control group and treatment groups.

p values less than 0.05.