Table 2. Best-fit average lifetimes (τ in ns) and energy transfer efficiencies (E ET, calculated from eqn (1)) at 293 K and 310 K for the excited state quenching of 2 by 32+ at different 32+ mole fractions in PEGylated (4 mol%) DMPC vesicles, as measured with transient absorption (TA) spectroscopy and time-correlated single photon counting (TCSPC). All liposomes had a fixed amount of compound 2 (0.5 mol%). Transient absorption spectroscopy data was globally fitted using the software package Glotaran.50 Full datasets are given in ESI, as well as detailed information on data analysis.
| Molar percentage of 32+ in PEGylated DMPC liposomes |
||||||
| 0.0 | 0.4 | 0.8 | 1.7 | 2.5 | 3.3 | |
| TA spectroscopy at 293 K | ||||||
| τ | 6.00 ± 0.10 | 3.03 ± 0.02 | 1.52 ± 0.01 | 1.04 ± 0.01 | 0.40 ± 0.00 | 0.29 ± 0.00 |
| E ET (%) | 0 | 50 | 75 | 83 | 93 | 95 |
|
32+ molar percentage in PEGylated DMPC liposomes |
||||||
| 0.0 | 0.5 | 0.9 | 1.7 | 2.4 | 3.5 | |
| TCSPC at 293 K | ||||||
| τ | 6.17 ± 0.01 | 4.67 ± 0.01 | 2.50 ± 0.05 | 1.29 ± 0.02 | 1.25 ± 0.05 | 0.85 ± 0.06 |
| E ET (%) | 0 | 24 | 60 | 79 | 80 | 86 |
| TCSPC at 310 K | ||||||
| τ | 5.69 ± 0.01 | 4.00 ± 0.01 | 2.37 ± 0.07 | 1.14 ± 0.00 | 0.84 ± 0.00 | 0.45 ± 0.00 |
| E ET (%) | 0 | 30 | 58 | 80 | 85 | 92 |