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. 2015 Nov 12;5:16539. doi: 10.1038/srep16539

Figure 1. Rab32 Facilitates the Efficiency of Reprogramming.

Figure 1

(a) Schematic representation of iPSC generation with different factor combinations. (b,d) The reprogramming efficiencies of factor combinations on the induction of iPSCs were quantified by determining the number of Oct4-GFP+ colonies in mES medium (b) and 10% KOSR medium (d) n = 4, the results of OSK+Ctr were used as control. *p < 0.05; **p < 0.01 by general linear model. (c,e) Full-well mosaic images of Oct4-GFP+ are shown for OSKCtr, OSKR and OSKN at day 16 and AP+ colonies at day 18 in mES medium (c) and 10% KOSR medium (e). (f) Characterization of Oct4-GFP+ dynamics for OSKCtr (blue plot) and OSKR (red plot) based on live imaging. (g) Representative of Oct4-GFP+ colonies during reprogramming (bar = 2 mm). (h,i) Reprogramming efficiency was increased by Rab32 in TF4 MEFs. (j,k) Rab32 knockdown reduces the number of AP+ colonies following the induction of iPSCs in TF4 MEFs. Data in (f,h,j) are represented as M ± SD (n = 3). The results of TF4 + Ctr, TF4 + shCtr and OSKCtr were used as controls respectively. *p < 0.05.