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. 2015 Nov 12;5:16552. doi: 10.1038/srep16552

Figure 1. A positive reciprocal regulation between TGF-β1 and CD147 in HSC activation.

Figure 1

(a) Real-time RT-PCR and (b) western blot analysis of CD147, Sp1, α-SMA, collagen I, and MMP-2 stimulated with various doses of TGF-β1 for 24 hours. (c) Real-time RT-PCR analysis of CD147, TGF-β1, α-SMA, and α1(I) collagen in LX-2 cells transiently transfected with plasmid pcDNA3.1-CD147 at 24 hours. (d) ELISA detection of total and active forms of TGF-β1 in supernatant of LX-2-transfected pcDNA3.1-CD147 cells. Cells were transfected with pcDNA3.1(+) as a mock control. (e) Western blot analysis of CD147, α-SMA, collagen I, and TGF-β1 in LX-2-CD147 cells treated with siRNA targeting CD147 (si-CD147). snc-RNA was used as a control. *P < 0.05, **P < 0.01, ***P < 0.001.