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. 2015 Nov 12;5:16552. doi: 10.1038/srep16552

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(a) Western blot analysis of CD147, p-Smad2, Smad2, p-Smad3, Smad3, and Smad4 expressions in LX-2 cells stimulated with various doses of TGF-β1 for 24 hours. (b) Immunofluorescent staining of Smad4 in LX-2 cells stimulated with TGF-β1. Cell nuclei were stained with DAPI. Western blot analysis of CD147 expression in LX-2 cells which were transfected with siRNA targeting Smad2 (c), Smad3 (d), and Smad4 (e) in the presence of 4 ng/ml TGF-β1 for 24 hours. snc-RNA was used as control.