Skip to main content
. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Neurobiol Dis. 2015 Jun 30;82:281–288. doi: 10.1016/j.nbd.2015.06.017

Fig.3.

Fig.3

Early recruitment of UBQLN2 to inclusions of HD-KI (Q200) mice but not SCA3-KI mice. (A) Double immunostaining for ubiquitin (green) and UBQLN2 (red) was performed in hippocampus of 19, 45 and 75 week old heterozygous HD-KI (Q200) mice. The presence of ubiquitin and UBQLN2 immunoreactivity within HTT nuclear inclusions paralleled the timing of HTT inclusion formation in the hippocampus. Scale bars = 10 μm. (B) Double immunostaining for ATXN3 (green) and UBQLN1/2 (red) was performed in hippocampus of 50 and 100-week-old homozygous SCA3-KI mice; the anti-UBQLN antibody used in B recognizes both UBQLN1 and UBQLN2. Even in 2-year old SCA3-KI mice, UBQLN1 and UBQLN2 do not localize to SCA3 intranuclear inclusions. (C) Double immunofluorescence for ATXN3 (green) and UBQLN2 (red) in the CA1 of hippocampus of 100-week-old SCA3-KI mice and age-matched wild type mice showing that UBQLN2 is not recruited into SCA3 nuclear inclusions even at an advanced age. Scale bars = 10 μm. (D) Double immunofluorescence for ATXN3 (green) and UBQLN2 (red) in the striatum radiatum (SR) of the hippocampus from 100-week-old SCA3-KI mice and age-matched wild type mice showing that UBQLN2 is not recruited into large extranuclear ATXN3 inclusions.