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| Items | Setting |
|---|---|
| Chromatographic Column | Gemini C18 (4.6 × 250, 5 μm) |
| Mobile Phase (v/v) | Acetonitrile: 0.05% trifluoroacetic acid = 4:6 |
| Detecting Wavelength | 214 nm |
| Temperature | 30 °C |
| Speed | 1 ml/min |
| Time | 20 min |
| Sample Size | 20 μl |
