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. 2015 Nov 12;5:16674. doi: 10.1038/srep16674

Figure 1.

Figure 1

(A) Sections of knee articular cartilage from healthy (n = 10) and OA (n = 10) patients stained with anti-IL-36α (20X). Representative sections and the quantification of immunostaining are shown. IL-36α mRNA expression was also determined by qRT-PCR in human primary chondrocytes (B,C). Basal expression of IL-36α and IL-36R, evaluated by qRT-PCR in human primary chondrocytes, immune cells attached to cartilage, PBMCs, SW1353 cells, TC28 cells, synovial tissues and infrapatellar fat pad (n = 5). Amplicons were electrophoresed on 2% agarose gel, stained with ethidium bromide and visualized with a high definition CCD camera. Glyceraldehyde-3 phosphate dehydrogenase (GAPDH) expression is also shown. CHON = chondrocytes; INFILT = immune cells infiltrated into the joint; PBMC = peripheral blood mononuclear cells; SW1353 = SW1353 human chondrosarcoma cell line; TC28 = T/C-28a2 human chondrocyte cell line; SIN = synovial tissue; IFP = infrapatellar fat pad.