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. 2015 Nov 12;5:16674. doi: 10.1038/srep16674

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(A–C) Determination of human MMP-13, NOS2 and COX-2 mRNA and protein expression by qRT-PCR and western blot respectively after 24 hours recombinant IL-36α or IL-1β treatment in human primary chondrocytes. (D) Determination of the degradation of IκB and the phosphorylation of p38 by western blot. β-actin and total p38 were used to confirm equal load. The results shown were obtained of at least three independent experiments, using at least three OA articular chondrocytes independent cultures. Low panels. Data showing densitometric analysis of all performed western blots.