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. 2015 Nov 12;5:16437. doi: 10.1038/srep16437

Figure 4. Knockdown of A20 overcomes tumor-induced T-cell tolerance.

Figure 4

(a,b) Analysis of antigen-specific T cell response after the treatment of si-RNA and PBS. T cells isolated from lymph nodes in different groups of mice were subjected to IFN-γ intracellular staining after the re-stimulation with OT-I peptide (a) or OT-II peptide (b) (n = 3). Total number of 30000 cells was analyzed. Cells were gated by CD4+ or CD8+ region. Percentages of the positive cells are illustrated. Numbers illustrated indicate the percentage of the cells in total cells. (c) Lymphocytes of draining lymph nodes from immunized mice were subjected to Foxp3 intracellular staining (n = 3). Total number of 30000 cells was analyzed. Cells were gated by CD4+. Numbers illustrated indicate the percentage of the cells in total cells. Data are representative of two independent experiments (n = 3). Data represent means ± SD. *p < 0.05 compared to si-SCR (ANOVA test).