Table 2. Primers for transcriptome sequencing and qRT-PCR.
| Genes | Primers for transcriptome sequencing (5′→3′)a | Length (bp) | Primers for qRT-PCR (5′→3′)b | Length (bp) |
|---|---|---|---|---|
| LOX5 | P1:GCCAAGAACACCCGTATCCC | 760 | F:TCGCGTCTACCGTTACGACTACTA | 180 |
| P2:GGCTTCAGCGTGCCATCGTC | R:TTCAGGTTCAGCAGGAAAAGC | |||
| BBTI2 | P1:CAAGGACCCGATTATCAGCA | 327 | F:TGGGACTGCTGCGACTTCG | 135 |
| P2:CCAGGATAGCCGTGGAAGAC | R:GCATCGGTAGCCAGGAGGG | |||
| BBTI11 | P1:CAGCACTCTGTTGGCGATCC | 239 | F:GCCGACGACGAAGAAGCA | 95 |
| P2:CGCAGTTTTCGGGGAGGAAG | R:GCCGACGACGAAGAAGCA | |||
| BBTI12 | P1:GGTGCTGATCCTGTGCCTCCA | 213 | F:GGCTAAAGAAAATGCCCTGGTT | 200 |
| P2:ACGGCGACGACGCACTTCTTG | R:CGGTGCACCTGAACTTGTTG | |||
| BBTI13 | P1:GCACGAGGAGGAGGAGGAGGAA | 221 | F:TTCGCCGTCCTCGTAGCT | 150 |
| P2:GGCGCTTGCAGAAGTTGACGAT | R:TCCCTGCACTCGCATATCG | |||
| CI-1B | P1:CGTGGAGGACGCCAAGAAGGT | 116 | F:AAGAAGGTGATCCTCAAGGACAAG | 100 |
| P2:CGAAGATGCGGACACGGTTAG | R:AAGATGCGGACACGGTTAGG | |||
| TPS10 | P1:AAGCCATTACCTTCACCA | 626 | F:ATCTCACCCTTCAAACCCC | 168 |
| P2:TCCTCGGACTAACACCTT | R:TAACCTCTTTCAACTCCTCAC | |||
| DOX | P1:GGAAGAACATCCCAACCTA | 755 | F:GGATTCGTTTTCGTTCATGCAT | 473 |
| P2:CTGCCATAAGACCAACAAG | R:TCCGGTGCGGTACATGAACT | |||
| A20/AN1 | P1:ACCCATCCTCTGCATCAATA | 344 | F:CTGGCTGCCTCCTCTATTGACA | 197 |
| P2:ATCCCGTAAGTCCAACCCTC | R:TTCGGCCCTACCTTCCCC | |||
| GAPDH | — | P1:CACGGCCACTGGAAGCA | ||
| — | P2:TCCTCAGGGTTCCTGATGCC | |||
| P450-72A1 | P1:CAGCACATCGCCTTCTCCACCATTT | 652 | F:CGCCTTCTCCACCATTTTCAA | 178 |
| P2:TCGTCACCCCGTTCTCGAACCTCTC | R:GAGCCCGAGCAGGTCGTTCCC | |||
| CYP72A5 | P1: TTTGGGAGCAACTATCAAGAAGGG | 870 | F: GGTGCTCAGATAAGGCTCAA | 186 |
| P2: GTGCAGTGTTATCACGGTGTACGG | R: ACATCGCAATGCATACAAGG | |||
| P450-a | P1: GCATGTCCCCCGTCATCTGC | 995 | F: TCGCCGTGCTGCTCTCTTTGC | 180 |
| P2: TCCATCAACCGTGTCCCAGT | R: CGATCTCTGCCTGTGCCTTCT | |||
| P450-c | P1: GCTTCAGCGAGGGGAGGAGGATTTT | 839 | F: AGAGGCATCGTCAGCAAGCG | 178 |
| P2: GTGAGTGTAGGACTGCGAGAGGGCG | R: TCCCGGCGAAGTAGAACAGC | |||
| P450-e | P1: ACAAACCACACACCCCACCTACCCC | 541 | F: ACGCTGTGGTCGATCGAGTG | 149 |
| P2: GTCCTTCCTCACGTCCTCCACCACC | R: ATGGCCTGAAGGTAGGGGAG | |||
| P450-g | P1: CGACGCAACTGAACCGATCGATGGA | 705 | F: TACAAGAAGCGGGGAGAGGAA | 137 |
| P2: TGACGGCAGGGAGGAAGGCGAAGAC | R: TCGGAGCATAGGAGGATAATC | |||
| AN17 | P1: TCCTCTCGTCGCTCGCTCATCACCC | 333 | F: CCCAATCAATCGCTAACAA | 159 |
| P2: CGCCCTGCCCCGGAACCAGCCTCTC | R: AGTCGAAGGGCAGGAAGTC | |||
| GPX | P1: CTCGGCTTCTTCTACACGCTAC | 360 | F: ACAGCCGTAAACGCCCCTCCC | 143 |
| P2: ACTGGACAATCTCCTCATTGGT | R: TCAACGTCTTTGCCGCTCGCA |
Notes:
aP1 stands for forward primers, and P2 stands for reverse primers for transcriptome sequencing.
bF stand for forward primers, and R stands for reverse primers for qRT-PCR analysis. GAPDH (Accession no. NM_001111943) is an internal reference gene for qRT-PCR.