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. 2015 Aug 7;5:13144. doi: 10.1038/srep13144

Figure 3. The expression of DBC1 and AR are associated with the signals for the proliferation and invasion of cells.

Figure 3

(a,b) Western blotting indicates that the protein levels of BCL-2, TGFβ, RhoA, NFκB, and PCNA were significantly decreased and the expression of P21 and P27 were significantly increased with the knock-down of DBC1 by siRNA in the both U2OS (wild-type P52) and SaOS2 (P53-null) cells. In U2OS cells, acetylation of P53 was significantly increased with the knock-down of DBC1. Especially, the protein level of AR was significantly decreased with the knock-down of DBC1. (c) However, the mRNA level of AR did not decrease with the knock-down of DBC1 as demonstrated by quantitative reverse-transcription PCR. (d,e) The knock-down of AR increased the protein levels of P21, P27, and BAX, and decreased the protein levels of TGFβ, RhoA, and NFκB in the both U2OS and SaOS2 cells. But the protein level of DBC1 (e) and mRNA level of DBC1 (f) were not significantly changed with the knock-down of AR with siRNA for AR. The statistical analysis for the quantification of protein expression was performed from three western blots. *p < 0.05, **p < 0.001.