Figure 2. Phylogenetic analysis of the rrs gene of representative bacterial transconjugant isolates recovered on M10 and M2SC based media following metaparental mating.

Isolates recovered on M10 and M2SC medium are indicated in blue and green typeface respectively while similar isolates recovered on both types of media are indicated in red typeface. The isolates were identified on the basis of partial sequencing of the rrs gene and the presence of the vector was confirmed by PCR and/or vector rescue. Representative microbial isolates and reference sequences are shown in bold black typeface.