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. 2015 Aug 21;5:13282. doi: 10.1038/srep13282

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(a) Analysis of vector specific PCR products (circa 1.4 kb) amplified from putative transconjugants bearing recombinant vectors. The PCRs were performed on individual transconjugants isolated on M2SC medium with primers targeting the region carrying the erythromycin resistance gene and oriT modules. (b). Analysis of undigested pEHR512111 vectors (linear vector circa 3.2 kb) rescued from individual transconjugants strains isolated on M2SC medium and prepared from E. coli DH10B-T1^R.