Figure 4. The effects of the individual mutations of several conserved residues putatively participating in substrate binding on the growth-supporting and 2-NBDG-transporting capabilities of Stp1.

(a) Growth curves of yeast cells expressing the respective variants as indicated on 1% glucose. (b) Fluorometric analysis of 2-NBDG uptake by yeast cells expressing the respective variants after a 4-h incubation with 2-NBDG. The averaged readout of the control experiments with WT Stp1 is set as 100%. Values in the panels are the mean of three biological replicates. Error bars are the SD from these replicates.