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. 2015 Sep 8;5:13829. doi: 10.1038/srep13829

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(a,b) Growth curves of yeast cells expressing the respective variants of Phe 50 as indicated with 1% glucose as the sole carbon source. (c) HPLC analysis of the residual glucose in the culture supernatants of the yeast cells expressing the respective variants. (d) Fluorometric analysis of 2-NBDG uptake by the respective variants, obtained by measuring the fluorescence intensity of the yeast transformants incubated with 2-NBDG for the indicated time period. Values in all the panels are the mean of three biological replicates.